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KMID : 0351619690100020081
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Kyungpook Medical Journal
1969 Volume.10 No. 2 p.81 ~ p.96
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Studies on Metacercaria-induced Rabbit Clonorchiasis
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Abstract
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It is the purpose of this paper to study the pathological and biochemical changes of the metacercaria-induced rabbit clonorchiasis with emphasis on the therapeutic effect of the anti-clonorchial agent, 2,2-methylenebis (3,4,6 tri-chlorophenoxyacetic acid) (MTPA).
Thirty-three rabbits, with an average body weight of 2 kg., were divided into 2 groups.
Group Ⅰ. Eighteen rabbits were subdivided into 6 groups: A,B,C,D,E and F. Subgroup A is a control. Subgroup B through F were infested through the mouth with 600 metacercariae of C. sinensis, collected from the muscle of the silver carp, Pseudorasbora parva, from the pond in the vicinity of Taegu. The infestation intervals between metacercarial administration and sacrificing animals for the subgroup B,C,D,E and F were, 4,8,10,12 and 16 weeks, respectively.
Group Ⅱ. Fifteen rabbits were subdivided into 5 groups: A,B,C,D and E. Subgroup A was infested with metacercariae of C. sinensis for 8 weeks. No anti-clonorchial agent was administered and this served as a control group. To the subgroup 3, 20mg./kg. of MTPA, per day, for 1 week, was administered orally, strating on the 8th infestation week. To the remainder of the subgroups, this was a standard therapeutic regimen, and therefore, all subgroups were treated identically with this anti-clonorchial agent, except the control group. The time between termination of anti-clonorchial agent administration and sacrificing animals was: immediate, 1,3 and 7 weeks for B.C.D and E, respectively.
Weekly variations of the total serum protein, albumin, electrophoretic fractionation of globulin, bilirubin, cholesterol, alkaline phosphatase, thymol turbidity and serum transaminases for all animals were carried out, while pathological examinations were performed on the sacrificed animals of their brain, heart, lung, liver, kidney, spleen, pancreas, adrenal, small intestine, testis and ovaries.
The reulsts were as follows:
Group Ⅰ.A mild, moderate, considerable, advanced and far advanced cirrhotic change was observed in subgroups, B,C,D,E and F order. This liver change correlates well with infestation time lapse. In the far advanced cirrhotic rabbits found in group F, ascites and congestive splenomegaly were also present. Other visceral organs were unremarkable. Serum chemistry showed a marked fall in serum albumin at the end of 2nd week whereas A/G ratio dropped during 4th week, both serum albumin and A/G ratio persisted to the end of this experiment with continuing low level. Significant rise was noted in beta-globulin cholesterol and alkaline phosphatase, all of which persisted within those high level to the end; while the transient rise in serum transaminases returned to normal at the end of 5th week. The thymol turbidity did not respond to the liver damage well and kept a low figure throughout.
Group Ⅱ. After 8 weeks of standard treatment with MTPA those cirrhotic rabbits in subgroud F which had shown a moderate to considerable degree of cirrhotic change were found to be restored to normal. This was not only proven by pathological study but by biochemical study also. The abnormal serum chemistry noted in group Ⅰ returned to normal after treatment with the aforementioned anti-clonorchial agent.
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